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A plasmid system (pMRX‐IP‐GFP‐LC3‐RFP‐LC3ΔG) was transfected to mesothelioma cells for detection of macroautophagy. Western blots using a green fluorescent protein (GFP) antibody confirmed light chain 3 (LC3) expression in mesothelioma cells after plasmid introduction compared to cells without the plasmid. β‐actin, internal control.
