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. Author manuscript; available in PMC: 2021 Feb 14.
Published in final edited form as: Methods Mol Biol. 2016;1436:147–165. doi: 10.1007/978-1-4939-3667-0_11

Fig. 1.

Fig. 1

Total and conditional HDAC11KO genotyping. Three genotyping primers were specifically designed to identify the loxP sites downstream and upstream of exon 3 and one primer found within the exon. Schematic representations of the three genotyping primer locations on a C57BL/6 wild-type HDAC11 allele (a), a conditional HDAC11KO allele (b), and a total HDAC11KO allele are displayed above. Genotyping PCR will result in a 437 bp fragment for a wild-type allele (a), a 471 bp fragment for a loxP allele (b), and a 278 bp fragment for a total HDAC11KO allele (c)