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. Author manuscript; available in PMC: 2021 Feb 14.
Published in final edited form as: Methods Mol Biol. 2016;1436:147–165. doi: 10.1007/978-1-4939-3667-0_11

Fig. 4.

Fig. 4

ChIP analysis of HDAC11 in IL-10 promoter region. HDAC11 is recruited to the IL-10 gene promoter in macrophages under LPS stimulation. Macrophages were treated with LPS at 1.0 g/mL, and then harvested at 0 or 0, 0.5, 1, 2, 3, 5, and 7 h after treatment. Cells were then harvested and proceed to ChIP analysis using anti-HDAC11 cocktail and anti-rabbit IgG as described. Quantitative real-time PCR analysis was performed in the −87 base pair to −7 base pair region of the mouse gene IL-10 promoter. The recruitment of HDAC11 to IL-10 promoter was observed at 0.5 h and reached to a peak by 2 h after LPS stimulation [8] (representative figure from previously published studies)