Table 3.

Universal touchdown genotyping PCR

Step	Temperature (°C)	Time	Actions per step
1	94	3 minutes
2	94	30 seconds
3	70	30 seconds	Decrease 0.5 °C per cycle
4	72	30 seconds
5			Go to step 2 40 times
6	94	30 seconds
7	50	30 seconds
8	72	30 seconds
9			Go to step 6 15 times
10	72	2 minutes
11	4	Infinite	Hold