Table 4.
Flow cytometry master mixes
| A | uL per test | CD Marker or Designation | Fluorescent molecule | C | uL per test | CD Marker or Designation | Fluorescent molecule |
|---|---|---|---|---|---|---|---|
| Common Myeloid Progenitor | 1 | CD3 | eFluor 450 | Monocytes (Classical and Non-Classical) | 1 | CD3 | eFluor 450 |
| 1 | CD19 | v450 | 1 | CD19 | v450 | ||
| 1 | NK1.1 | v450 | 1 | NK1.1 | v450 | ||
| 1 | NKG2D | v450 | 1 | NKG2D | v450 | ||
| 1 | CD11b | v450 | 1 | CD115 | APC | ||
| 1 | Ly6g | v450 | 1 | Ly6c | AlexaFlour700 | ||
| 1 | CD45 | PE-Cy7 | 1 | CD43 | PE | ||
| 1 | CD34 | AlexaFlour700 | 1 | MHCII | PE-Cy7 | ||
| 1 | CD127 | PerCP-Cy5.5 | 8uL per well | ||||
| 1 | CD117 (c-Kit) | APC | |||||
| 10uL per well | |||||||
| B | uL per test | CD Marker or Designation | Fluorescent molecule | D | uL per test | CD Marker or Designation | Fluorescent molecule |
| Dendritic Cells | 1 | CD3 | eFluor 450 | Granulocytes (Neutrophils/Eosinophils) | 1 | CD3 | eFluor 450 |
| 1 | CD19 | v450 | 1 | CD19 | v450 | ||
| 1 | NK1.1 | v450 | 1 | NK1.1 | v450 | ||
| 1 | NKG2D | v450 | 1 | NKG2D | v450 | ||
| 1 | CD11b | PE | 1 | CD11b | APC | ||
| 1 | CD11c | APC | 1 | Ly6g | PE | ||
| 1 | CD4 | PerCP | 6uL per well | ||||
| 1 | CD8 | AlexaFlour700 | |||||
| 8uL per well | |||||||
See Note 7