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Quantitative RT-PCR
| Step | Temperature (°C) | Time | Actions per step |
|---|---|---|---|
| 1 | 95 | 3 minutes | |
| 2 | 95 | 30 seconds | |
| 3 | 60 | 30 seconds | Read |
| 4 | Go to step 2 40 times | ||
| 5 | 95 | 1 minute | |
| 6 | 58 | 10 seconds |
Melt Curve (Start Temp.) Read 0.5°C/cycle to End Temp. |
| 7 | 95 | Melt curve (End Temp.) |
