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. 2021 Feb 10;13:1257–1270. doi: 10.2147/CMAR.S290645

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© 2021 Huo and Dou.

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Expression and validation of circ_0056285 in OS cells. (A) The expression of circ_0056285 in hFOB1.19 and OS cell lines (143B, MG63, U2OS and HOS) was measured by qRT-PCR. (B) The schematic illustration showed the origin of circ_0056285 and the result of Sanger sequencing. (C) Circ_0056285 was amplified by divergent primers in cDNA but not gDNA. (D) 143B and HOS cells were treated with Actinomycin D, and the expression of circ_0056285 and GAPDH was examined by qRT-PCR at different times. (E) The levels of circ_0056285 and GAPDH were detected in 143B and HOS cells treated with or without RNase R. (F) The levels of 18S rRNA, U6 and circ_0056285 in nuclear and cytoplasmic fractions were evaluated by qRT-PCR. **P < 0.01, ***P < 0.001.