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. 2021 Feb 10;13:1257–1270. doi: 10.2147/CMAR.S290645

Figure 2.

Figure 2

Circ_0056285 knockdown inhibited proliferation and promoted apoptosis in OS cells. 143B and HOS cells were introduced with si-NC, si-circ#1 or si-circ#2. (A) The knockdown efficiency was assessed by qRT-PCR. (B and C) Cell proliferative capacity was evaluated by CCK-8 assay and colony formation assay. (D) Cell apoptosis was detected by flow cytometry. (E and F) The activities of caspase 3 and caspase 9 were examined using commercial kits. ***P < 0.001.