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. 2021 Feb 10;13:1257–1270. doi: 10.2147/CMAR.S290645

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© 2021 Huo and Dou.

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Circ_0056285 directly interacted with miR-1244. (A) Venn diagram showed miRNAs bound to circ_0056285 in Circbank and circinteractome databases. (B) The levels of four miRNAs (miR-1244, miR-548b-3p, miR-604 and miR-614) were tested in 143B and HOS cells transfected with si-NC or si-circ#1. (C) MiR-1244 expression in hFOB1.19 and OS cell lines (143B, MG63, U2OS and HOS) was evaluated using qRT-PCR. (D) The expression of miR-1244 was tested in 143B and HOS cells transfected with miR-1244 or miR-NC. (E) The predicted binding sites of circ_0056285 and miR-1244 were exhibited. (F) 143B and HOS cells were co-transfected with WT-circ_0056285 or MUT-circ_0056285 and miR-1244 or miR-NC, and luciferase activity was detected by dual-luciferase reporter assay. (G and H) The interaction between circ_0056285 and miR-1244 was further verified using RIP assay and RNA pull-down assay. **P < 0.01, ***P < 0.001.