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. 2021 Feb 10;13:1257–1270. doi: 10.2147/CMAR.S290645

Figure 6.

Figure 6

Inhibition of miR-1244 or overexpression of TRIM44 reversed the effect of circ_0056285 depletion on OS progression. (A) The expression of miR-1244 was detected in 143B and HOS cells transfected with anti-NC or anti-miR-1244. (B) TRIM44 protein level was examined in 143B and HOS cells introduced with vector or oe-TRIM44. (C–M) 143B and HOS cells were transfected with si-NC, si-circ#1, si-circ#1+anti-NC, si-circ#1+anti-miR-1244, si-circ#1+vector or si-circ#1+oe-TRIM44, respectively. (C and D) Cell viability was assessed by CCK-8 assay. (E) The number of colonies was detected by colony formation assay. (F) Cell apoptosis was monitored by flow cytometry. (G and H) Caspase 3 and caspase 9 activities were tested by commercial kits. (I–K) After 48 h of transfection, glucose uptake, lactate product and ATP level were detected. (L and M) Western blot assay was utilized to detect the protein levels of HK2 and LDHA. *P < 0.05, **P < 0.01, ***P < 0.001.