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. 2021 Jan 15;141(3):431–453. doi: 10.1007/s00401-020-02257-0

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© The Author(s) 2021

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Fig. 5 — Western blot assessing soluble expression of WT and missense variant-containing titin domains. Bacterial cultures expressing His-tagged titin domains were lysed, separated into total and soluble fractions, run on Western blot and probed with anti-His tag antibody