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Construction of Innolysins Cj. Innolysin Cj1 was constructed by fusing the C-terminus of phage T5 endolysin to the tail fiber protein H of Campylobacter CAMSA2147 CJIE1-like prophage. These two domains were fused by using a linker in between composed of 14 amino acids (GAGAGAGAGAGAGA). The same configuration was used for Innolysin Cj2 but instead of the whole H-fiber, the C-terminal part of the H-fiber was used, excluding the N-terminal DUF3751 domain.
