TABLE 1.
Bacterial strains.
Description | References | |
P. aeruginosa strains | ||
PAO1 | Clinical wound isolate; R2-pyocin producer | Williams et al., 2008 |
PAO1 Δprf15 | P. aeruginosa PAO1 lacking the R2-pyocin tail fiber due to an in-frame deletion of codons 11–301 of prf15 | Williams et al., 2008 |
PEG02 | PAO1 Δprf15/pM63, campycin + chaperone co-expression strain, Gmr (15 μg/ml) | This study |
PEG16 | PAO1 Δprf15/pM96, campycin expression strain, Gmr (15 μg/ml) | This study |
E. coli strains | ||
BL21-CodonPlus (DE3)-RIL | Contains a ColE1-compatible, pACYC-based plasmid containing extra copies of the argU, ileY, and leuW tRNA genes and was used as a protein expression strain, Camr (50 μg/ml) | Agilent Technologies |
InCj1 | BL21-CodonPlus (DE3)-RIL/pAZInCj1, Innolysin Cj1 + chaperone co-expression strain, Kanr (100 μg/ml), Camr (50 μg/ml) | This study |
InCj2 | BL21-CodonPlus (DE3)-RIL + pAZInCj2, Innolysin Cj2 + chaperone co-expression strain, Kanr (100 μg/ml), Camr (50 μg/ml) | This study |
InCj5 | BL21-CodonPlus (DE3)-RIL + pAZInCj5, Innolysin Cj5 + chaperone co-expression strain, Kanr (100 μg/ml), Camr (50 μg/ml) | This study |
AZE1 | BL21-CodonPlus (DE3)-RIL/pAZE1, T5 endolysin expression strain, Kanr (100 μg/ml), Camr (50 μg/ml) | This study |
AZE5 | BL21-CodonPlus (DE3)-RIL/pAZE5, Salmonella phage Shivani peptidase expression strain, Kanr (100 μg/ml), Camr (50 μg/ml) | This study |
B195 | BL21-CodonPlus (DE3)-RIL/CRYS3, C-terminal of H-fiber + chaperone co-expression strain, Kanr (100 μg/ml), Camr (50 μg/ml) | This study |
C. jejuni strains | ||
NCTC11168 | A preferred strain for studying phage-host interactions at the molecular level | National collection of type cultures |
NCTC11168 ΔkpsMΔmotA | A capsular and non-motile flagellated NCTC11168 mutant | Baldvinsson et al., 2014 |