TABLE 3.
Plasmids used or constructed.
| Plasmid | Construction | Used for | References |
| pUCPtac | Modified pUCP30T (Gmr broad-host-range plasmid, Accession no: U33752) containing laclq tac MCSa | Cloning vector | Williams et al., 2008 |
| pET-28a (+) | N-His, N-Thrombin, C-His, Kanr (100 μg/ml) | Expression vector | Novagen |
| pM50 | pUCPtac:N-terminus of prf15 of R2-pyocin lacking the binding domain, Gmr (15 μg/ml) | Engineering R2-pyocins | This study |
| pM63 | pM50:C-terminus of H-fiber gene + chaperone gene of CAMSA2147 CJIE1, Gmr (15 μg/ml) | Campycin co-expression with chaperone | This study |
| pM96 | pM50:C-terminus of H-fiber gene of CAMSA2147 CJIE1, Gmr (15 μg/ml) | Campycin expression | This study |
| pAZE1 | pET-28a (+):T5lys from phage T5, Kanr (100 μg/ml) | T5 endolysin expression | This study |
| pAZE5 | pVTD: Salmonella phage Shivani peptidase gene, Kanr (100 μg/ml) | Salmonella phage Shivani peptidase expression | This study |
| pCRYS3 | pET-28a (+):C-terminus of H-fiber gene + chaperone gene of CAMSA2147 CJIE1, Kanr (100 μg/ml) | H-fiber expression | This study |
| pAZInCj1 | pET-28a (+):Innolysin Cj1 + chaperone gene of CAMSA2147 CJIE1, Kanr (100 μg/ml) | Innolysin Cj1 expression | This study |
| pAZInCj2 | pET-28a (+):Innolysin Cj2 + chaperone gene of CAMSA2147 CJIE1, Kanr (100 μg/ml) | Innolysin Cj2 expression | This study |
| pAZInCj5 | pVTD:Innolysion Cj5 + chaperone of CAMSA2147 CJIE1, Kanr (100 μg/ml) | Innolysin Cj5 expression | This study |
aMCS, multiple cloning site.