Fig. 1.

Analysis workflow. Individual neurons were isolated from postmortem human hippocampal brain tissue by laser microdissection (LMD). Using immunohistochemistry three populations of neurons were visualized and isolated with high accuracy: 1. Control neurons, from cognitively healthy control cases that were negative for CK1δ and pTau (AT8) immunoreactivity, 2. granulovacuolar degeneration (GVD) bearing neurons, as recognised by the typical CK1δ positive granular staining pattern, and 3. pTau positive neurons, mostly tangle-bearing neurons, were isolated from AD cases. For each neuronal population 3000 individual neurons were isolated per case. The protein content of each sample was then analysed using mass spectrometry (a). Peptide level statistics were applied to identify proteins that are differentially expressed in neurons with GVD and neurons with pTau compared to control neurons (b). All resulting proteins are listed in table S2. The subset of proteins which are increased in GVD are listed in Table 3