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. 2021 Feb 1;9:623829. doi: 10.3389/fcell.2021.623829

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Copyright © 2021 Álvarez-Satta, Lago-Docampo, Bea-Mascato, Solarat, Castro-Sánchez, Christensen and Valverde.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALMS1 knockdown increases the number of aberrant cilia and ciliary length. (A) Representative immunofluorescence image of primary cilia in untransfected cells. Primary cilia (arrows) are stained in red, ALMS1 protein (asterisks) in green and cell nuclei in blue (DAPI). A zoomed cilium is also shown. (B) Percentage of ciliated cells in control (siMock) and silenced (siALMS1) cells (n = 3). (C) Representative immunofluorescence images of primary cilia in control and silenced cells showing different aberrant morphologies (n = 3). (D) Dot plot representing each of the measurements of ciliary length for control and silenced cells; n = 3 with at least 50 cilia measured per biological replicate. (E) Radar plot of the percentage of ciliary twisting and its bending angles between control and silenced cells (n = 3). Error bars represent mean ± SD (*p < 0.05, **p < 0.01).