Figure 1.
Tamoxifen treatment suppresses expression of Arp2/3 complex subunits. (A,B) Western blot analysis of Arp2/3 complex subunit levels in individual Actr3fl/fl clones after tamoxifen treatment (72, 96, or 120 h) or DMSO/EtOH used as vehicle control. GAPDH and vinculin served as loading controls, dependent on the molecular weight of explored Arp2/3 complex subunit. (A) Although the extent of Arp3 protein decrease correlated with prolonged tamoxifen treatment, a treatment of 96 h (red box) was determined as best compromise between efficient protein run down and cell viability, which was significantly compromised upon 120 h (not shown). The 96 h-time point was thus used for all future experiments. (B) Tamoxifen-induced removal of Arp3 (96 h) concomitantly reduced all additional Arp2/3 complex subunits tested, as indicated.