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. 2021 Feb 1;9:634708. doi: 10.3389/fcell.2021.634708

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Copyright © 2021 Dimchev, Lahmann, Koestler, Kage, Dimchev, Steffen, Stradal, Vauti, Arnold and Rottner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Arp3-depleted MEFs are devoid of lamellipodia. (A) Phalloidin-stained examples of distinct Actr3^fl/fl clones (Arp3.5, Arp3.7, and Arp3.19) stably expressing tamoxifen-inducible Cre recombinase, with (tamoxifen) or without (DMSO/EtOH) tamoxifen induction (96 h) causing disruption of the Actr3 gene. Tamoxifen treatment causes near to complete elimination of lamellipodia in representative cells shown. Scale bars as indicated in individual panels. Note the strong increase of spread cell area upon Arp3 disruption. (B) Quantification of lamellipodia formation efficiency upon treatments as shown in A. Data are arithmetic means and standard errors of means from three independent experiments; n = total number of cells analyzed; data were statistically compared using two-sided, two-sample t-test (***p < 0.001).