Figure 5.

Acute Arp2/3 complex removal reduces wound closure capacity. (A) Selected frames from wound healing, time-lapse microscopy (24 h) of control- and tamoxifen-treated Actr3^fl/fl clones. Wounds closed on average after roughly 18 h in controls, but failed to do so in Arp3 null cells. (B) Quantification of collective migration velocity during wound closure utilizing movies as displayed in (A). Bar charts show arithmetic means and SEMs. n = number of movies analyzed, generated from three independent experiments. Differences in wound closure speed between individual control- and tamoxifen-treated clones were confirmed to be statistically significant using two-sided two-sample t-test (***p < 0.001).