Table 1.
List of primers used in the current study.
| Gene Name* | Primer sequence (5'---3') | Product size (bp) | Annealing temperature (°C) | Genome reference |
|---|---|---|---|---|
| GAPDH | GCTAAGGCTGTGGGGAAAGT TCAGCAGCAGCCTTCACTAC |
161 | 56 | NM_204305.1 |
| RUNX2 | TCTCTGAACTCTGCACCAAGTC GCTCGGAAGCACCTGAGAGG |
229 | 58 | NM_204128.1 |
| COL1A2 | CTGGTGAAAGCGGTGCTGTT CACCAGTGTCACCTCTCAGAC |
222 | 57 | NM_001079714.2 |
| SPP1 | GCCCAACATCAGAGCGTAGA ACGGGTGACCTCGTTGTTTT |
204 | 57 | NM_204535.4 |
| BMP2 | TCAGCTCAGGCCGTTGTTAG GTCATTCCACCCCACGTCAT |
163 | 57 | XM_025148488.1 |
| BGLAP | GACGGCTCGGATGCTCGCAG CAGACGGGGCCGTAGAAGCG |
227 | 57 | NM_205387.3 |
| ALP | CGACCACTCACACGTCTTCA CGATCTTATAGCCAGGGCCG |
140 | 58 | NM_205360.1 |
| PPAR-γ | GAGCCCAAGTTTGAGTTTGC TCTTCAATGGGCTTCACATTT |
131 | 58 | XM_025154400.1 |
| FASN | AGAGGCTTTGAAGCTCGGAC GGTGCCTGAATACTTGGGCT |
127 | 58 | NM_205155.3 |
| FABP4 | GCAGAAGTGGGATGGCAAAG GTTCGCCTTCGGATCAGTCC |
153 | 58 | NM_204290.1 |
| MYOD1 | GGATGCATACTACCCAGTG GCTGTCGTAGCTGTTTCT |
136 | 58 | NM_204214.2 |
| MYF5 | GAGAGGCAGCAGCTTCGAG GTCCACGATGCTGGAGAGG |
109 | 58 | NM_001030363.1 |
| MYOG | AGCAGCCTCAACCAGCAGGA TCTGCCTGGTCATCGCTCAG |
179 | 58 | NM_204184.1 |
| CYP24A1 | TAATGACGGCCCTACTGCTG AGTCCTTCTGCTGCGCTAAA |
271 | 58 | NM_204979.1 |
*
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RUNX2, runt related transcription factor 2; COL1A2, collagen type I alpha 2 chain; SPP1, secreted phosphoprotein 1; BMP2, bone morphogenetic protein 2; BGLAP, bone gamma-carboxyglutamate protein; ALP, alkaline phosphatase; PPAR-γ, peroxisome proliferator-activated receptor gamma; FASN, Fatty acid synthase; FABP4, fatty acid binding protein 4; MYOD1, myogenic differentiation 1; MYF5, myogenic factor 5; MYOG, myogenin; CYP24A1, cytochrome P450 family 24 subfamily A member 1.