Table 1.

Purification of recombinant protein MxynB-8, XynB-DT, Xln-DT, and Dt-xyl3.

Enzyme	Culture extract			Ni affinity chromatography			Yield (%)	Fold purification
	Total activity (U)	Total protein (mg)	Specific activity (U/mg)	Total activity (U)	Total protein (mg)	Specific activity (U/mg)
MxynB-8	56,312.8	97.7	576.38	43,923.9	48.2	910.68	78.1	1.58
XynB-DT	13,766.1	2,935.2	4.69	9,553.7	352.2	27.12	69.4	5.78
Xln-DT	560.4	190.5	2.94	318.3	37.2	8.56	56.8	2.91
Dt-xyl3	532.4	287.9	1.85	285.4	46.3	6.16	53.4	3.35

Substrate for MxynB-8 and XynB-DT was beechwood xylan, while substrate for Xln-DT and Dt-xyl3 was p-nitrophenyl-β-d-xylopyranoside.