Table 3.
Kinetic parameters and specific activities of recombinant Xln-DT and Dt-xyl3.
| Substrate | Xln-DT | Dt-xyl3 | ||||
|---|---|---|---|---|---|---|
| Km (mM) | Vmax (U/mg) | Relative activity (%) | Km (mM) | Vmax (U/mg) | Relative activity (%) | |
| pNPX | 1.66 | 13.58 | 100.0 | 0.83 | 19.05 | 100.0 |
| pNPG | 3.67 | 6.03 | 25.9 | 2.39 | 10.90 | 21.6 |
| oNPG | ND | ND | ||||
| pNPGal | ND | ND | ||||
| pNPR | ND | ND | ||||
| pNPA | 1.02 | 3.89 | 44.4 | 2.04 | 28.68 | 30.8 |
| Cellobiose | 11.00 | 217.39 | — | 2.28 | 277.78 | — |
| Xylobiose | 0.93 | 714.29 | — | 4.18 | 666.7 | — |
| CMC | ND | ND | ||||
pNPX, p-nitrophenyl-β-d-xylopyranoside; pNPG, pNP-β-d-glucoside; oNPG, oNP-β-d-glucoside; pNPGal, p-nitrophenyl-β-d-galactopyranoside; pNPR, p-nitrophenyl-α-l-rhamnopyranoside; pNPA, pNP-α-l-arabinofuranoside; CMC, carboxymethylcellulose; ND, not detected.
Values shown are the mean of duplicate experiments, and the variation about the mean is below 5%.