Table 4.

Simultaneous or sequential hydrolysis reactions by endo-1,4-β-xylanase MxynB-8 and β-xylosidase Xln-DT against poplar sawdust xylan substrate.

Enzyme added				Poplar sawdust xylan
First reaction	Reaction conditions	Second reaction	Reaction conditions	Xylose concentration (μmol)	Synergy
MxynB-8	50°C, pH 6.0, dosage of 500 U/g, 18 h	None	—	26.94	—
MxynB-8+Xln-DT	50°C, pH 6.0, dosage of 500 and 100 U/g, 18 h	None	—	322.54	2.03
MxynB-8+Xln-DT	80°C, pH 6.0, dosage of 500 and 100 U/g, 18 h	None	—	433.48	3.07
MxynB-8	50°C, pH 6.0, dosage of 500 U/g, 18 h	Xln-DT	80°C, pH 6.0, dosage of 100 U/g, 4 h	1,798.57	15.89
Xln-DT	80°C, pH 6.0, dosage of 100 U/g, 4 h	MxynB-8	50°C, pH 6.0, dosage of 500 U/g, 18 h	958.58	8.01
Xln-DT	80°C, pH 6.0, dosage of 100 U/g, 4 h	None	—	79.50	—

Simultaneous or sequential reactions refer to the reactions with two enzymes added simultaneously or sequentially, respectively.

Synergy degree is defined as the ratio of xylose equivalents from simultaneous or sequential enzyme combinations to the sum of that released by the individual enzymes.