Table 2.
Comparison of RT-qPCR and RT-RAA/LFD results for clinical samples.
No. | RT-qPCR result (Ct value) | RT-RAA/LFD | ||
---|---|---|---|---|
Orf1ab | N-gene | E-gene | Judgment | |
1 | 32.97 | 32.8 | 31.64 | + |
2 | 27.29 | 26.92 | 25.89 | + |
3 | 33.52 | 33.75 | 32.09 | + |
4 | 31.23 | 30.87 | 29.65 | + |
5 | 33.15 | 33.04 | 32.10 | + |
6 | 35.89 | 34.60 | 33.97 | + |
7 | 28.04 | 27.41 | 26.56 | + |
8 | 21.75 | 21.98 | 22.15 | + |
9 | 27.00 | 26.44 | 26.21 | + |
10 | 30.78 | 31.10 | 30.56 | + |
11 | 39.05 | 37.51 | 38.10 | + |
12 | 34.95 | 35.27 | 35.18 | + |
13 | 21.38 | 21.16 | 20.51 | + |
Mean | 30.54 | 30.22 | 29.59 |
RT-qPCR and RT-RAA/LFD represent quantitative real-time polymerase chain reaction, reverse-transcription recombinase-aided amplification coupled with lateral flow dipstick, respectively.