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. 2021 Feb 1;10:540332. doi: 10.3389/fonc.2020.540332

Table 1.

Summary of N-linked glycans released from HeLa, HeLa/ST3GalIV and SiHa, SiHa/ST3GalIV cell lines in MALDI-TOF-MS.

Peak Observed m/z Calculated m/za ) Chemical compositionb）
No. HeLa HeLa/ST3GalⅣ SiHa SiHa/ST3GalⅣ
1 1579.48 1579.65 1579.49 1579.60 1579.78 (Hex)2 + (Man)3(GlcNAc)2
2 1590.50 1590.67 1590.51 1590.63 1590.80 (HexNAc)1 (Fuc)1 + (Man)3(GlcNAc)2
3 1620.51 1620.68 1620.55 1620.63 1620.81 (Hex)1 (HexNAc)1 + (Man)3(GlcNAc)2
4 1783.62 1783.82 1783.65 1783.77 1783.88 (Hex)3 + (Man)3(GlcNAc)2
5 1794.65 1794.84 1794.68 1794.78 1794.90 (Hex)1 (HexNAc)1 (Fuc)1 + (Man)3(GlcNAc)2
6 1824.67 1824.87 1824.69 1824.82 1824.91 (Hex)2 (HexNAc)1 + (Man)3(GlcNAc)2
7 1835.69 1835.89 1835.73 1835.84 1835.92 (HexNAc)2 (Fuc)1 + (Man)3(GlcNAc)2
8 1987.81 1988.03 1987.85 1987.97 1987.98 (Hex)4 + (Man)3(GlcNAc)2
9 1998.83 1999.04 1998.89 1998.98 1999.00 (Hex)2 (HexNAc)1 (Fuc)1 + (Man)3(GlcNAc)2
10 2028.85 2029.08 2028.90 2029.03 2029.01 (Hex)3 (HexNAc)1 + (Man)3(GlcNAc)2
11 2039.87 2040.09 2039.93 2040.05 2040.02 (Hex)1 (HexNAc)2 (Fuc)1 + (Man)3(GlcNAc)2
12 2069.89 2070.11 2069.94 2070.07 2070.03 (Hex)2 (HexNAc)2 + (Man)3(GlcNAc)2
13 2080.93 2081.14 2080.96 2081.06 2081.05 (HexNAc)3 (Fuc)1 + (Man)3(GlcNAc)2
14 2155.96 2156.19 2156.00 2156.13 2156.07 (Hex)1 (HexNAc)1 (Fuc)1 (NeuAc)1 + (Man)3(GlcNAc)2
15 2191.98 2192.22 2192.03 2192.16 2192.08 (Hex)5 + (Man)3(GlcNAc)2
16 2244.04 2244.28 2244.11 2244.23 2244.12 (Hex)2 (HexNAc)2 (Fuc)1 + (Man)3(GlcNAc)2
17 2360.09 2360.35 2360.15 2360.27 2360.17 (Hex)2 (HexNAc)1 (Fuc)1 (NeuAc)1 + (Man)3(GlcNAc)2
18 2390.10 2390.36 2390.17 2390.29 2390.18 (Hex)3 (HexNAc)1 (NeuAc)1 + (Man)3(GlcNAc)2
19 2396.10 2396.37 2396.16 2396.30 2396.18 (Hex)6 + (Man)3(GlcNAc)2
20 2418.13 2418.39 2418.19 2418.32 2418.21 (Hex)2 (HexNAc)2 (Fuc)2 + (Man)3(GlcNAc)2
21 2431.13 2431.39 2431.19 2431.32 2431.21 (Hex)2 (HexNAc)2 (NeuAc)1 + (Man)3(GlcNAc)2
22 2519.16 2519.42 2519.23 2519.33 2519.26 (Hex)3 (HexNAc)3 + (Man)3(GlcNAc)2
23 2564.14 2564.41 2564.20 2564.34 2564.27 (Hex)3 (HexNAc)1 (Fuc)1 (NeuAc)1 + (Man)3(GlcNAc)2
24 2605.15 2605.42 2605.21 2605.35 2605.30 (Hex)2 (HexNAc)2 (Fuc)1 (NeuAc)1 + (Man)3(GlcNAc)2
25 2693.14 2693.41 2693.21 2693.34 2693.35 (Hex)3 (HexNAc)3 (Fuc)1 + (Man)3(GlcNAc)2
26 2779.07 2779.37 2779.15 2779.29 2779.39 (Hex)2 (HexNAc)2 (Fuc)2 (NeuAc)1 + (Man)3(GlcNAc)2
27 2792.07 2792.35 2792.13 2792.27 2792.38 (Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2
28 2879.97 2880.29 2880.06 2880.21 2880.44 (Hex)3 (HexNAc)3 (NeuAc)1 + (Man)3(GlcNAc)2
29 2965.87 2966.18 2965.94 2966.10 2966.47 (Hex)2 (HexNAc)2 (Fuc)1 (NeuAc)2 + (Man)3(GlcNAc)2
30 2995.83 2996.14 2995.91 2996.06 2996.48 (Hex)3 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2
31 3053.74 3054.06 3053.81 3053.98 3054.52 (Hex)3 (HexNAc)3 (Fuc)1 (NeuAc)1 + (Man)3(GlcNAc)2
32 3141.56 3141.89 3141.66 3141.81 3142.58 (Hex)4 (HexNAc)4 (Fuc)1 + (Man)3(GlcNAc)2
33 3227.35 3227.68 3227.42 3227.60 3228.61 (Hex)3 (HexNAc)3 (Fuc)2 (NeuAc)1 + (Man)3(GlcNAc)2
34 3240.32 3240.65 3240.39 3240.57 3239.63 (Hex)1 (HexNAc)4 (Fuc)3 (NeuAc)1 + (Man)3(GlcNAc)2
35 3328.06 3328.40 3328.13 3328.31 3327.65 (Hex)2 (HexNAc)2 (Fuc)1 (NeuAc)3 + (Man)3(GlcNAc)2
36 3413.77 3414.11 3413.84 3414.02 3413.72 (Hex)1 (HexNAc)4 (Fuc)4 (NeuAc)1 + (Man)3(GlcNAc)2
37 3501.44 3501.80 3501.52 3501.70 3501.74 (Hex)2 (HexNAc)2 (Fuc)2 (NeuAc)3 + (Man)3(GlcNAc)2
38 3587.08 3587.43 3587.12 3587.32 3587.81 (Hex)1 (HexNAc)4 (Fuc)5 (NeuAc)1 + (Man)3(GlcNAc)2
39 3600.02 3600.36 3600.10 3600.26 3600.80 (Hex)1 (HexNAc)4 (Fuc)3(NeuAc)2 + (Man)3(GlcNAc)2
40 3773.09 3773.47 3773.18 3773.38 3772.30 (Hex)2 (HexNAc)6 (NeuAc)2 + (Man)3(GlcNAc)2
41 3860.57 3860.97 3860.66 3860.87 3860.94 (Hex)3 (HexNAc)7 (NeuAc)1 + (Man)3(GlcNAc)2
42 3948.00 3948.41 3948.10 3948.31 3948.99 (Hex)4 (HexNAc)8 + (Man)3(GlcNAc)2
43 4218.84 4219.30 4218.95 4219.19 4219.10 (Hex)7 (HexNAc)3 (Fuc)3 (NeuAc)1 + (Man)3(GlcNAc)2
44 4306.10 4306.51 4306.21 4306.40 4307.15 (Hex)8 (HexNAc)4 (Fuc)3 + (Man)3(GlcNAc)2
45 4576.21 4576.68 4576.34 4576.57 4576.26 (Hex)6 (HexNAc)3 (NeuAc)4 + (Man)3(GlcNAc)2

a) The glycans were calculated as permethylated glycan and [M + Na]+.

b) Monosaccharide compositions were determined by database searching using GlycoMod (http://www.expasy.ch/tools/glycomod/).

Monosaccharides are indicated as follows: Hex, hexose; HexNAc, N-acetyl hexosamine; Fuc, fucose; NeuAc, N-acetyl neuraminic acid; Man, mannose.