FIGURE 2.

A schematic depicting the in vitro exposure system (IVES) connected to air and smoke sources. A three-way valve connects the cannabis cigarette to IVES through a 50-mL syringe. Another three-way valve connects room air to IVES. Smoke is drawn through the smoke exposure syringe and expelled with predetermined rate and volume into IVES. Room air is introduced with the fresh air syringe in a similar fashion. A heating pad positioned below IVES maintains the experimental system at 37°C (figure generated with BioRender).