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LPS-induced acute inflammation in porcine PBMCs. (a–c) TNF-α, IL-6 and IL-1β mRNA expression were determined by qRT-PCR in porcine PBMCs at 4, 8, 12 and 24 h after treatment with or without LPS. (d) The concentration of TNF-α in supernatants of the PBMCs was measured at 4, 8, 12 and 24 h after treatment with or without LPS. The data represent the mean ± SD. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001 vs. control.
