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. 2020 Nov 26;27(2):118–132. doi: 10.1177/1753425920966645

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© The Author(s) 2020

Creative Commons CC BY: This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 8. — Role for SEMA7A in monocyte differentiation into pro-inflammatory macrophages. (a) Bright field microscope images from THP-I monocytes and macrophages. (b) Representative immunoblot analysis of SEMA7A or ACTB (loading control) in protein lysates of THP-I monocytes and macrophages. (c) Bright field microscope images from THP-I macrophages transduced with anti-SEMA7A shRNA (sh-SEMA7A) or scrambled shRNA (sh-Ctrl). Elongated phenotype indicated by #. (d) Ratio of TNF-α and IL-10 mRNA expression of sh-SEMA7A or sh-Ctrl THP-I macrophages. Data are the mean ± SEM; n = 4; *P < 0.05.