Table 3.
Overview of regulated genes and their known relevant function.
| Gene | Mono-mϕ RNA-seq | Mono-mϕ microarray | THP-1 microarray | Expression | Type | Annotation |
|---|---|---|---|---|---|---|
| NTNG2 | Down | Down* | – | 11 | Netrin ligand | Function unknown |
| ADORA2B | Up | – | Up* | 111 | Netrin receptor | ADORA2B has a pro-inflammatory role through production of cAMP22 |
| EPHA1 | Down | Down* | Down* | 11 | Ephrin receptor | EPHA1 is a receptor for EFNA1, which promotes monocyte adhesion23 |
| EPHB3 | Down | Down* | – | 1 | Ephrin receptor | EPHB3 is a receptor for EFNB2, which promotes monocyte adhesion24 |
| EPHB4 | Down | Down* | Down* | 1. | Ephrin receptor | EPHB4 is a receptor for EFNB2, which promotes monocyte adhesion24 |
| SEMA3C | Up | – | Up* | 11111 | Semaphorin | SEMA3C enhances deformability of DCs, promoting migration of DCs25 |
| SEMA4A | Down | Down | Down | 111111 | Semaphorin | SEMA4A promotes expression of VEGFA in monocytes26 |
| SEMA4B | Down | Down* | – | 1111 | Semaphorin | Function unknown |
| SEMA4D | Down | Down* | Down* | 11111. | Semaphorin | AntiSEMA4D treatment augments pro-inflammatory function of mϕ27 SEMA4D itself also promote differentiation of monocyte to alternatively activated mϕ28 |
| SEMA4F | Up | Up* | Up* | . | Semaphorin | Function unknown |
| SEMA6A | Down | – | Down* | 1. | Semaphorin | SEMA6A signals via PLXNA4 leading to repulsion of cell migration29 |
| SEMA7A | Up | Up* | Up* | 1 | Semaphorin | SEMA7A works as a stimulator of monocytes, promoting the production of GM-CSF3 |
| NRP1 | Up | Up* | Up* | 11 | Neuropilin | NRP1 is a co-receptor of Class III semaphorins, which are repellents for monocyte adhesion and migration4 NRP1+ mϕ migrate towards SEMA3A expressed in hypoxic niches of tumours30 |
| NRP2 | Up | Up* | Up* | 11 | Neuropilin | NRP2 is a co-receptor of Class III semaphorins, which are repellents for monocyte adhesion and migration.4 NRP2 is required for phagocytosis of mϕ31 |
| PLXNA1 | Up | Up* | Up* | 11 | Plexin | PLXNA1 is a receptor of Class III semaphorins, which are repellents for monocyte adhesion and migration4 PLXNA1 facilitates migration of tumour-associated mϕ into hypoxic niche30 |
| PLXNA3 | Up | Up* | Up* | 1. | Plexin | PLXNA3 is a receptor of Class III semaphorins, which are repellents for monocyte adhesion and migration4 |
| PLXND1 | – | Up* | Up* | 11111 | Plexin | SEMA3E-PLXND1 axis promotes mϕ infiltration in adipose tissue32 |
Annotation of functions of the NGCs that showed consistent changes in at least two of the three monocyte-to-macrophage differentiation data sets.
*P < 0.05).
mϕ: macrophages; DC: dendritic cells; NGC: neuronal guidance cue; Expression: expression level indicated with 111111 being highest and lowest.