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. 2021 Jan 28;21(4):239. doi: 10.3892/ol.2021.12500

Figure 5.

Figure 5.

Effect of hsa_circ_0000129 on EZH2 expression. (A) RT-qPCR and (B) western blot analyses demonstrated that EZH2 expression was significantly upregulated in the cancer cell lines compared with normal breast cells. (C) RT-qPCR and (D) western blot analyses demonstrated that overexpression of hsa_circ_0000129 upregulated EZH2 expression in MCF-7 cells. (E) RT-qPCR and (F) western blot analyses demonstrated that hsa_circ_0000129 knockdown downregulated EZH2 expression in MDA-MB-468 cells. **P<0.01, *** P<0.001. Circ, circular; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; si, small interfering.