FIGURE 5.

Neferine inhibited IL‐13‐induced autophagy activation by up‐regulating Nrf2 expression in GO orbital fibroblasts. GO orbital fibroblasts were treated with IL‐13 (10 ng/mL) in the presence or absence of neferine (500 nM) for 7 days. (A) Representative immunofluorescence images of LC3 puncta (magnification: 40×). (B–G) Western blot analysis and densitometric quantification of the expression of autophagy‐related proteins. Data indicate the mean ± SD (n = 5). *P < .05, ***P < .001 vs control; ^## P < .01, ^### P < .001 vs IL‐13