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. 2020 Dec 30;25(4):2228–2237. doi: 10.1111/jcmm.16215

FIGURE 4.

FIGURE 4

Huaier reverses the energy metabolism in A549 via inhibiting PI3K/ AKT/HIF‐1α signalling pathway. Huaier (4 mg/mL), EGF (10 ng/mL) and Wortmannin (2 μg/mL) were used to investigate the possible mechanism of Huaier reversal on energy metabolism. (A‐B) EGF significantly increased the protein expression of key enzymes for glucose metabolism, while Wortmannin showed the opposite results; (C‐D) silencing of HIF‐1α reversed the energy metabolism in A549 cells. HK‐II, Hexokinase II; PFK, phosphofructokinase; PK, pyruvate kinase; GLUT1, glucose transporter 1; PI3K: phosphatidylinositol 3‐kinase; HIF‐1α, hypoxia‐inducible factor‐1; EGF, epidermal growth factor. *Compared with Huaier group, P < .05