Figure 5.

Combination of HG and OxLDL decreases Nrf2 levels, activity and translocation in the nucleus. HUVECs were cultured and treated as in Figure 3, then examined for Keap1 expression (A) as well as Nrf2 expression and phosphorylation (B) in Western blot and quantified in densitometry analysis. Endothelial marker CD31 (red) and Nrf2 (green) immunofluorescence were examined following the indicated treatments (C). Results are represented as mean ± SEM, n = 4 in each treatment group. *P < .05, **P < .01, ***P < .001