TABLE 1.

Gene expression changes on plaque CD68⁺ cells

Apoe^−/− and APOA1^tg/tg/Apoe^−/− male and female mice were fed Western diet for 12 or 16 weeks, respectively. After this period, a subset of mice was sacrificed (baseline), and the rest of the mice were fed chow diet (control diet) or chow diet containing 900 mg/kg of the ACAT inhibitor F1394 (ACAT inhibitor diet for 4 extra weeks. Total mRNA was isolated from CD68⁺ cells in atherosclerotic lesions using LCM on lesions present at the level of the aortic root. The expressions of proinflammatory gene markers and anti-inflammatory gene markers are represented. Hypoxanthine-guanine phosphoribosyltransferase was used as housekeeping control gene.

Genotype	Group (sample size)	Proinflammatory markers (mean ± S.D.)			Anti-inflammatory markers (mean ± S.D.)
		CCL2	NLRP3	IL-1β	FIZZ1	MRC1	CD36
Apoe−/− mice	Baseline (7)	1.00 ± 0.91	1.00 ± 0.65	1.00 ± 1.14	1.00 ± 1.39	1.00 ± 1.22	1.00 ± 0.75
	Control diet (5)	1.09 ± 0.50	2.07 ± 2.35	0.57 ± 0.54	0.02 ± 0.02	0.41 ± 0.50	2.03 ± 0.80
	ACAT inhibitor diet (6)	1.06 ± 1.73	1.01 ± 1.54	0.32 ± 0.34	0.01 ± 0.01	0.32 ± 0.28	2.35 ± 1.43
APOA1tg/tg/Apoe−/− mice	Baseline (7)	1.82 ± 1.34	1.09 ± 2.29	0.42 ± 0.41	0.02 ± 0.02	0.99 ± 0.93	1.49 ± 1.01
	Control diet (7)	0.56 ± 0.57	0.94 ± 2.09	0.35 ± 0.19	1.14 ± 2.77	0.59 ± 0.41	2.51 ± 1.27
	ACAT inhibitor diet (7)	0.38 ± 0.54	0.02 ± 0.01	0.07 ± 0.10	10.66 ± 15.49	2.97 ± 3.44	3.11 ± 1.77