Figure 5. SARS‐CoV‐2 infects human organoid‐derived 2D bronchioalveolar‐like cultures.

• A, B
  Infectious virus titers can be observed by virus titrations on VeroE6 cells of apical washes at 2, 24, 48, and 72 h after infection of bronchioalveolar‐like cells from donor 1 (A) and donor 2 (B) at MOI 0.01 (red) or 0.1 (black) with SARS‐CoV‐2. The dotted line indicates the lower limit of detection. Error bars represent SEM. N = 4 for all except donor 2 MOI 0.1, which is N = 3. H p.i. = hours post‐infection.
• C–E
  Immunofluorescent staining of SARS‐CoV‐2‐infected differentiated bronchioalveolar‐like cultures. Nucleoprotein (NP) stains viral capsid (red), which colocalized with HOPX (C), HTII‐280 (D), and SFTPC (E). Scale bars indicate 50 μm. Nuclei are stained with Hoechst and shown in white (C‐E).