Figure 7. Low‐dose interferon lambda 1 treatment dramatically reduces SARS‐CoV‐2 replication in bronchioalveolar‐like cultures.

• A, B
  SARS‐CoV‐2 replication shown as infectious virus titers (A) and viral RNA copies (B) in apical washes at 2, 24, 48, and 72 h after infection at MOI 0.1. Cultures were either pretreated with 50 ng/ml IFN lambda 1 in the basal compartment 2 h prior to the infection (green), at 24 h p.i. or left untreated. The dotted line indicates the lower limit of detection. Error bars represent SEM. N = 2. H p.i. = hours post‐infection.
• C
  Whole well immunofluorescent staining of representative cultures in (A) and (B) using an anti‐NP antibody (red). Nuclei are stained with Hoechst (blue). Scale bar indicates 1 mm.