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. 2021 Feb 1;12:620934. doi: 10.3389/fphar.2021.620934

FIGURE 4.

FIGURE 4

Oxidative stress played a crucial role in crizotinib- and sunitinib-induced hepatotoxicity in vitro and in vivo. (A) L02 cells were incubated with various concentrations of crizotinib or sunitinib for 48 h (n = 3). ROS levels were analyzed using a ROS assay kit. (B, C) Total and nuclear Nrf2 levels in L02 cells were measured. (D) C57 mice were gavaged with 100 mg/kg/day crizotinib or 120 mg/kg/day sunitinib for 14 days (n = 6). The levels of GPx, MDA and SOD were measured to assess oxidative stress. (E) The mRNA levels of Nrf2, HO1 and NQO1 were determined by RT-qPCR (n = 8). (F, G) The hepatic protein levels of total and nuclear Nrf2 were determined by western blot analysis (n = 6). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control group.