Figure 1. Combining BN‐PAGE with IGX‐MS.

In the first step, proteins and their assemblies are loaded and separated by BN‐PAGE. The bands representing different protein assemblies are visualized after running the gel with Coomassie in the upper running buffer. The band(s) of interest are then excised from the gel and incubated with a cross‐linking reagent in the cross‐linking buffer. The cross‐linking reaction is quenched and subsequently subjected to standard in‐gel digestion. The extracted peptides are finally analyzed using cross‐linking optimized parameters for the MS analysis.