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. 2021 Jan 18;40(4):e106174. doi: 10.15252/embj.2020106174

Figure EV3. In‐solution XL‐MS of C6.

Figure EV3

  1. DSS concentration optimization for cross‐linking C6 in‐solution monitored by SDS–PAGE. The upper band (~200 kDa) indicates a C6 dimer that is formed upon cross‐linking, whereas the more abundant lower band (~100 kDa) represents monomeric C6.
  2. Obtained cross‐links for monomeric C6 plotted on the available X‐ray structure of C6 (PDB ID: 3T5O). The red lines indicate distances > 30 Å. The different domains of C6 are indicated in black.
  3. Distribution of lysine Cα‐Cα distances of unique cross‐links identified by in‐solution XL‐MS for monomeric C6 when plotted on the reported X‐ray structure (pink bars, PDB ID: 3T5O) and the IGX‐MS‐driven refined structural model (green bars). The average distance of all cross‐links is 41.7 Å when using the X‐ray structure which reduces to 26.2 Å for the IGX‐MS‐driven structural model.

Data information: Only cross‐links identified in at least two of three replicates were included in the analysis.