Figure EV4. ESM1 mainly induces nuclear translocation of β‐catenin (related to Fig 5).

• A
  Nuclear and cytosolic extracts of 22Rv1‐P and 22Rv1‐M cells were prepared, and the levels of indicated proteins were detected by immunoblotting.
• B
  Nuclear and cytosolic extracts of 22Rv1‐M cells transfected with either Scramble‐shRNA or ESM1‐shRNA were prepared, and the levels of indicated proteins were detected by immunoblotting.
• C
  Suppression of β‐catenin or NFκB by shRNA was analyzed.
• D, E
  Immunoblotting analysis of 22Rv1‐M cells transfected with either shScramble or shESM1 was performed with the antibodies indicated.
• F
  Nuclear and cytosolic extracts of 22Rv1‐M cells transfected with either shScramble or shRELA were prepared, and the levels of indicated proteins were detected by immunoblotting.
• G
  Graph derived from co‐expression analysis of published data available in the cBioPortal (EGAS00001002923 and phs000915.v1.p1). P‐value was obtained by two‐tailed Student’s t‐test.

Source data are available online for this figure.