Figure EV1. PIDD1 is recruited to the centriole distal appendage.

1. Schematic outlining the competition growth assay used to measure fold change in the number of GFP+ cells treated with doxycycline compared to control, untreated GFP− cells.
2. Graph showing the relative growth of doxycycline‐treated PLK4^Dox; TRIM37 ^−/− cells expressing an sgRNA targeting the indicated genes. Each dot displays measurements from a single experiment. Experiments were performed in polyclonal knockout cells. Data acquired across n ≥ 3 biological replicates. Mean ± s.e.m.
3. Representative images of PLK4^Dox RPE1 cells treated with and without doxycycline for two days and immunostained with the indicated antibodies. Scale bar = 5 µm.
4. Representative images of wild‐type and PIDD1 ^−/− PLK4^Dox RPE1 cells treated with and without doxycycline for two days and immunostained with the indicated antibodies. Scale bar = 5 µm.
5. Representative images of PLK4^Dox PIDD1‐mNeonGreen DLD1 cells treated with and without doxycycline for 2 days and immunostained with the indicated antibodies. Scale bar = 5 µm.
6. Representative images of WT or knockout PLK4^Dox cells immunostained with the indicated antibodies. Experiments were performed in PLK4^Dox monoclonal knockout cells. Scale bar = 5 µm.
7. Quantification of the fraction of cells with the indicated protein localized at the centriole. Experiments were performed in PLK4^Dox monoclonal knockout cells. Data acquired across n = 3 biological replicates. Mean ± s.e.m.
8. Representative images of WT or knockout PLK4^Dox cells immunostained with the indicated antibodies. Experiments were performed in PLK4^Dox monoclonal knockout cells. Scale bar = 5 µm.

Data information: Asterisks indicate statistically significant differences between measurements (*P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001). Statistics for all Figures were calculated using a two‐tailed Student’s t‐test.

Source data are available online for this figure.