Figure 3. ANKRD26 is required to arrest cell proliferation in response to centrosome amplification.

• A
  Quantification of the fraction of cells with ANKRD26 localized at the mature mother centriole. Experiments were performed in PLK4^Dox monoclonal knockout cells. Data acquired across n = 3 biological replicates. Mean ± s.e.m.
• B–D
  Representative images of WT or ANKRD26 ^−/− cells immunostained with the indicated antibodies. Scale bar = 5 µm.
• E
  Quantification of the fraction of WT or ANKRD26 ^−/− hTERT RPE1 cells with cilia. Each dot displays measurements from a single experiment. Data acquired across n = 5 biological replicates. Mean ± s.e.m.
• F
  Growth assay of the indicated cells with and without doxycycline‐inducible overexpression of PLK4. Experiments were performed in PLK4^Dox monoclonal knockout cells. Data acquired across n = 3 biological replicates. Mean ± s.e.m.
• G
  Quantification of centrosome number in PLK4^Dox cells expressing an sgRNA targeting the indicated genes. Experiments were performed in PLK4^Dox monoclonal knockout cells. Data acquired across n = 3 biological replicates. Mean ± s.e.m.
• H
  Western blot showing expression of pro‐CASP2 and P21 following treatment with dox for the specified number of days. Experiments were performed in PLK4^Dox monoclonal knockout cells.
• I
  Quantification of pro‐CASP2 levels following treatment with dox for the specified number of days. Experiments were performed in PLK4^Dox monoclonal knockout cells. Each dot displays measurements from a single experiment. Data acquired across n = 6 biological replicates. Mean ± s.e.m.

Data information: Asterisks indicate statistically significant differences between measurements (**P < 0.01; ****P < 0.0001). Statistics for all Figures were calculated using a two‐tailed Student’s t‐test.

Source data are available online for this figure.