Figure EV3. ANKRD26 is not required to activate the PIDDosome in response to DNA damage.

1. Western blot showing expression of pro‐CASP2 and P21 following treatment with etoposide. Experiments were performed in monoclonal PLK4^Dox knockout cells.
2. Quantification of pro‐CASP2 levels following treatment with etoposide. Experiments were performed in monoclonal PLK4^Dox knockout cells. Each dot displays measurements from a single experiment. Data acquired across n = 6 biological replicates. Mean ± s.e.m.
3. Western blot showing expression of pro‐CASP2 and P21 following treatment with etoposide. Experiments were performed in PLK4^Dox cells knocked out for the indicated genes.
4. Quantification of the fraction of proliferating cells following treatment with the indicated drugs/reagents. Experiments were performed in monoclonal PLK4^Dox knockout cells. Data acquired across n = 3 biological replicates. Mean ± s.e.m.

Data information: Asterisks indicate statistically significant differences between measurements (****P < 0.0001). Statistics for all Figures were calculated using a two‐tailed Student’s t‐test.

Source data are available online for this figure.