Figure EV4. An ANKRD26^ΔM2 mutant fails to recruit PIDD1 to the distal appendage.

1. Schematic representation of wild‐type ANKRD26 and various mutants.
2. Quantification of the fraction of cells with ANKRD26 localized to the mature mother centriole in monoclonal ANKRD26 ^−/− PLK4^Dox cells expressing the indicated mCherry‐ANKRD26 transgene. Each dot displays measurements from a single experiment. Data acquired across n ≥ 3 biological replicates. Mean ± s.e.m.
3. Quantification of the fraction of cells with PIDD1 localized to the mature mother centriole in monoclonal ANKRD26 ^−/− PLK4^Dox cells expressing the indicated mCherry‐ANKRD26 transgene. Each dot displays measurements from a single experiment. Data acquired across n = 3 biological replicates. Mean ± s.e.m.
4. Representative images of monoclonal ANKRD26 ^−/− PLK4^Dox cells expressing the indicated mCherry‐ANKRD26 transgene. Cells were immunostained with indicated antibodies. Scale bar = 5 µm.
5. Schematic of the co‐immunoprecipitation procedure performed in (F).
6. HEK293FT cells were transfected with the indicated constructs. Cell lysates were split and subjected to co‐immunoprecipitation with mCherry or GFP binder beads.
7. Quantification of the fraction of cells with PIDD1 localized to the mature mother centriole in monoclonal PIDD1 ^−/− PLK4^Dox cells expressing the indicated PIDD1 transgene. Each dot displays measurements from a single experiment. Data acquired across n = 3 biological replicates. Mean ± s.e.m.
8. Representative images of monoclonal PIDD1 ^−/− PLK4^Dox cells expressing the indicated PIDD1 transgene. Cells were immunostained with indicated antibodies. Scale bar = 5 µm.

Data information: Asterisks indicate statistically significant differences between measurements (^ns P > 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001). Statistics for all Figures were calculated using a two‐tailed Student’s t‐test.

Source data are available online for this figure.