Figure EV5. A recurrent ANKRD26 mutation is observed in human tumors.

1. Schematic showing the location of the 533 mutations in ANKRD26 in human tumors. Black represents truncating mutations; green represents missense mutations; purple represents inframe mutations. The K1234Nfs*19 mutation is shown in red. Data from curated set of non‐redundant studies in cBioPortal (Cerami et al, 2012).
2. Plot showing the distance between the closest CEP164 rings in cycling (EdU+) and non‐cycling (EdU− cells). Squares show the mean for each biological replicate; colored circles show individual data points from each of the replicates. Data acquired across n = 3, biological replicates, each with > 20 cells. P values, unpaired two‐tailed t‐test. Mean ± s.e.m.
3. Stacked bar graphs of the same data shown in (B). Mean ± s.e.m.
4. Representative images of PLK4^Dox RPE1 cells treated with and without doxycycline for two days and immunostained with the indicated antibodies. Scale bar = 5 µm.

Source data are available online for this figure.