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. 2020 Dec 22;40(4):e104844. doi: 10.15252/embj.2020104844

Figure EV2. Complementation of SCLT1 in SCLT1 KO cells rescues PIDDosome activation also in A549 cells.

Figure EV2

  1. Fluorescence micrographs of A549 cells of the indicated genotypes. Cells were either left untransduced (mock) or transduced with a lentiviral vector expressing Myc‐SCLT1 and co‐stained with the indicated antibodies. Blow‐ups without Hoechst 33342 are magnified 2.5×. Scale bar: 5 μm.
  2. Dot plot showing the average pixel intensities of PIDD1 at individual parent centrioles in A549 cells of the indicated genotypes, treated as in (A). Mean values (red lines) ± s.e.m. are reported. N > 50 centrosomes were assessed for each condition in as many individual cells; a.u. = arbitrary units. Mann–Whitney test (n.s. = non‐significant).
  3. A549 cells of the indicated genotypes were either left untransduced (−) or transduced with a lentiviral vector expressing Myc‐SCLT1 (+). Cells were treated either with DMSO or with ZM447439 for 24 h and subjected to immunoblotting; n = 3 independent experiments.
  4. DNA content analysis of A549 cells of the indicated genotypes. Cells were either left untransduced (mock) or transduced with a lentiviral vector expressing Myc‐SCLT1, treated with ZM447439 for up to 72 h and processed for DNA content analysis.

Source data are available online for this figure.