Skip to main content
. 2020 Dec 22;40(4):e104844. doi: 10.15252/embj.2020104844

Figure 5. PIDD1 autoproteolysis is constitutive, occurs independently of DAs localization but is necessary for PIDDosome activation.

Figure 5

  • A
    A549 cells of the indicated genotypes were either left untransduced or transduced with a lentiviral vector expressing PIDD1‐V5 in its wild‐type form. Cells were treated either with DMSO or with ZM447439 for 24h and subjected to immunoblotting. N = 2 independent experiments.
  • B, C
    A549 cells of the indicated genotypes were either left untransduced (mock) or transduced with lentiviral vectors expressing PIDD1‐V5 in its wild‐type form or carrying the indicated point mutations. Cells were treated either with DMSO or with ZM447439 for 24 h and subjected to immunoblotting. N = 2 independent experiments.
  • D
    Fluorescence micrographs of A549 cells of the indicated genotypes. Cells were either left untransduced (mock) or transduced with PIDD1‐V5 lentiviral vectors carrying the indicated point mutations and stained with the indicated antibodies. Blow‐ups without Hoechst 33342 are magnified 2.5×. Scale bar: 5 μm.

Source data are available online for this figure.