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. 2020 Dec 22;40(4):e104844. doi: 10.15252/embj.2020104844

Figure 7. Centrosome clustering is necessary for PIDDosome activation.

Figure 7

  1. Dot plot showing the distance between parent centrioles pairs in A549 cells following the indicated treatments (ZM = ZM447439; REV = reversine). Nocodazole concentrations are 0.03, 0.1, 0.33, 1, 3.3 µM. Median (red) and 95% confidence interval thereof (black) are shown. N > 50 cells were analysed.
  2. Immunoblot of A549 cells subjected to the indicated treatments for 24 h as in (A). N = 3 independent experiments.
  3. DNA content analysis of A549 cells subjected to the indicated treatments as in (A) either for 24 h (left panels) or 48 h (right panels). N = 2 independent experiments.
  4. Schematic of the experimental conditions utilized to synchronize RPE1 cells and to specifically interfere with centrosome clustering after telophase.
  5. Immunoblots of RPE1 cells synchronized as in (D). N = 3 independent experiments.
  6. Representative fluorescence micrographs of RPE1 cells synchronized as in (D). Centrosomal antigens were stained with the indicated antibodies. Blow‐ups without Hoechst 33342 are magnified 2×. Scale bar: 5 μm.

Source data are available online for this figure.