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. 2021 Jan 26;143(5):2423–2432. doi: 10.1021/jacs.0c13059

Figure 4.

Figure 4

(A) Fluorescent labeling of recombinant human saliva α-amylase (type XIII-A) with ABPs 1c, 2b, and 2c at pH 7 after 1 h incubation at 37 °C. (B) Fluorescent labeling of concentrated human saliva, mouse pancreas, and salivary gland lysates (55 μg total protein) with different concentrations of ABP 1c (pH 7.0, 37 °C). (C) pH-dependent labeling with ABP 1c in complex biological samples. The optimal pH is approximately 5.0 in these three samples. (D) Detection limit of α-amylase in complex biological samples labeled with ABP 1c (pH 5.0, 37 °C). (E) Time-dependent labeling of α-amylase in complex biological samples with ABP 1c (pH 5.0, 37 °C). (F) Competitive ABPP on amylases in extracts from human saliva (HS), mouse pancreas (MP), and mouse salivary gland (MSG), offset against Western blot detection of amylase abundance using an anti-amylase antibody.