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. 2021 Jan 26;143(5):2423–2432. doi: 10.1021/jacs.0c13059

Figure 5.

Figure 5

(A) Fluorescent labeling of day 5 A. nidulans secretome with ABP 1c at pH 3–8 after incubation for 1 h at 37 °C. (B) Temperature dependent labeling of day 5 A. nidulans secretome with ABP 1c after incubation at pH 5.0 for 1 h at 25–55 °C. (C) Fluorescent labeling of day 1–5 A. nidulans secretomes with ABP 1c at pH 5.0 after incubation for 1 h at 37 °C. (D) Competitive ABPP of day 5 A. nidulans secretome with acarbose. The secretome was preincubated with acarbose at varying concentrations (30 min, pH 5.0, 37 °C) prior to labeling with ABP 1c (67.5 μM, 1 h, pH 5.0, 37 °C). (E) Competitive ABPP of day 5 A. nidulans secretome with 3a. The secretome was preincubated with 3a at varying concentrations (30 min, pH 5.0, 37 °C) prior to labeling with ABP 1c (67.5 μM, 1 h, pH 5.0, 37 °C).